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goat anti mouse irf1 antibody (R&D Systems)

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R&D Systems goat anti mouse irf1 antibody

( A ) Mouse and ( B ) human networks hierarchical layouts after calculating all possible paths between LPS and IRG1 from the contextualised networks. <t>IRF1</t> , interferon regulatory factor 1; CEBPB, CCAAT/enhancer binding protein (C/EBP) beta; CEBPD, CCAAT/enhancer binding protein (C/EBP) delta; STAT1, signal transducer and activator of transcription 1; JUNB, Jun B proto-oncogene; PRDM1, PR domain containing 1 with ZNF domain; STAT4, signal transducer and activator of transcription 4; FOS, FBJ murine osteosarcoma viral oncogene homolog; ETS2, v-ets avian erythroblastosis virus E26 oncogene homolog 2; VDR, vitamin D (1,25-dihydroxyvitamin D3) receptor; RARA, retinoic acid receptor alpha; RUNX1, runt-related transcription factor 1. Solid line: activation; dashed line: inhibition.

Goat Anti Mouse Irf1 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+mouse+irf1+antibody/Mouse+IRF1+Antibody/pmc04752512-123-1-20
Average 90 stars, based on 3 article reviews

goat anti mouse irf1 antibody - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "Gene Regulatory Network Inference of Immunoresponsive Gene 1 ( IRG1 ) Identifies Interferon Regulatory Factor 1 ( IRF1 ) as Its Transcriptional Regulator in Mammalian Macrophages"

Article Title: Gene Regulatory Network Inference of Immunoresponsive Gene 1 ( IRG1 ) Identifies Interferon Regulatory Factor 1 ( IRF1 ) as Its Transcriptional Regulator in Mammalian Macrophages

Journal: PLoS ONE

doi: 10.1371/journal.pone.0149050

Figure Legend Snippet: ( A ) Mouse and ( B ) human networks hierarchical layouts after calculating all possible paths between LPS and IRG1 from the contextualised networks. IRF1 , interferon regulatory factor 1; CEBPB, CCAAT/enhancer binding protein (C/EBP) beta; CEBPD, CCAAT/enhancer binding protein (C/EBP) delta; STAT1, signal transducer and activator of transcription 1; JUNB, Jun B proto-oncogene; PRDM1, PR domain containing 1 with ZNF domain; STAT4, signal transducer and activator of transcription 4; FOS, FBJ murine osteosarcoma viral oncogene homolog; ETS2, v-ets avian erythroblastosis virus E26 oncogene homolog 2; VDR, vitamin D (1,25-dihydroxyvitamin D3) receptor; RARA, retinoic acid receptor alpha; RUNX1, runt-related transcription factor 1. Solid line: activation; dashed line: inhibition.

Techniques Used: Binding Assay, Virus, Activation Assay, Inhibition

Figure Legend Snippet: Transcription factors gene essentiality metric scores.

Techniques Used:

( A, B ) RAW264.7 cells were transfected with siRNA negative (siRNA NEG) or siRNA specific to Irf1 (siRNA Irf1) 24 hours before treatment and RNA was extracted 2 hours after activation with LPS (10ng/ml). The bars show the mean of 3 biological replicates (± SEM) of ( A ) Irf1 and ( B ) Irg1 mRNA levels measured by real-time PCR normalised with L27 as the housekeeping gene. **p < 0.01, *p < 0.05. ( C ) Proteins were extracted from transfected cells after 4 hours of LPS stimulation. Western blot bands of IRF1, IRG1/CAD and β-ACTIN proteins are shown. ( D, E ) Metabolites were extracted from transfected cells after 4h of LPS stimulation and analysed by GC-MS. Itaconic acid levels in Irf1 silenced cells were calculated as the percentage relative to the non-specific transfected cells in ( D ) control and ( E ) LPS activated cells. Error bars and statistical significance were calculated from 3 biological replicates (± SEM). *p < 0.05.

Figure Legend Snippet: ( A, B ) RAW264.7 cells were transfected with siRNA negative (siRNA NEG) or siRNA specific to Irf1 (siRNA Irf1) 24 hours before treatment and RNA was extracted 2 hours after activation with LPS (10ng/ml). The bars show the mean of 3 biological replicates (± SEM) of ( A ) Irf1 and ( B ) Irg1 mRNA levels measured by real-time PCR normalised with L27 as the housekeeping gene. **p < 0.01, *p < 0.05. ( C ) Proteins were extracted from transfected cells after 4 hours of LPS stimulation. Western blot bands of IRF1, IRG1/CAD and β-ACTIN proteins are shown. ( D, E ) Metabolites were extracted from transfected cells after 4h of LPS stimulation and analysed by GC-MS. Itaconic acid levels in Irf1 silenced cells were calculated as the percentage relative to the non-specific transfected cells in ( D ) control and ( E ) LPS activated cells. Error bars and statistical significance were calculated from 3 biological replicates (± SEM). *p < 0.05.

Techniques Used: Transfection, Activation Assay, Real-time Polymerase Chain Reaction, Western Blot, Gas Chromatography-Mass Spectrometry, Control

PBMCs-derived macrophages were transfected with siRNA negative (siRNA NEG) or siRNA specific to IRF1 (siRNA IRF1) 24 hours before treatment and RNA was extracted 6 hours after activation with LPS (10μg/ml) in independent donors (D1-D3). The bars show the mean of ( A ) IRF1 and ( B ) IRG1 mRNA levels of 3 technical replicates (± SEM) measured by real-time PCR normalised with L27 as the housekeeping gene. *p < 0.05.

Figure Legend Snippet: PBMCs-derived macrophages were transfected with siRNA negative (siRNA NEG) or siRNA specific to IRF1 (siRNA IRF1) 24 hours before treatment and RNA was extracted 6 hours after activation with LPS (10μg/ml) in independent donors (D1-D3). The bars show the mean of ( A ) IRF1 and ( B ) IRG1 mRNA levels of 3 technical replicates (± SEM) measured by real-time PCR normalised with L27 as the housekeeping gene. *p < 0.05.

Techniques Used: Derivative Assay, Transfection, Activation Assay, Real-time Polymerase Chain Reaction

Affinity Purification:

Article Title: Gene Regulatory Network Inference of Immunoresponsive Gene 1 ( IRG1 ) Identifies Interferon Regulatory Factor 1 ( IRF1 ) as Its Transcriptional Regulator in Mammalian Macrophages
Article Snippet: Heat-denatured protein samples (20μg) were separated on a 10% SDS-polyacrylamide gel electrophoresis followed by transfer to nitrocellulose membranes 0.2μm (Sigma). .. Affinity-purified goat anti-mouse IRF1 antibody (catalogue #: AF4715) and rabbit anti-goat IgG secondary antibody (catalogue #: HAF017) were obtained from R&D Systems Europe Ltd., United Kingdom, while rabbit anti-human IRG1 antibody (catalogue #: HPA040143) and normal rabbit IgG (catalogue #: sc-2027) were purchased from Sigma and Santa Cruz Biotechnology, respectively. .. Goat anti-β-actin (catalogue #: sc-1616) and anti-goat secondary antibodies (catalogue #: RPN1025) were purchased from Santa Cruz Biotechnology and GE Healthcare, respectively.

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Journal: PLoS ONE

Article Title: Gene Regulatory Network Inference of Immunoresponsive Gene 1 ( IRG1 ) Identifies Interferon Regulatory Factor 1 ( IRF1 ) as Its Transcriptional Regulator in Mammalian Macrophages

doi: 10.1371/journal.pone.0149050

Figure Lengend Snippet: ( A ) Mouse and ( B ) human networks hierarchical layouts after calculating all possible paths between LPS and IRG1 from the contextualised networks. IRF1 , interferon regulatory factor 1; CEBPB, CCAAT/enhancer binding protein (C/EBP) beta; CEBPD, CCAAT/enhancer binding protein (C/EBP) delta; STAT1, signal transducer and activator of transcription 1; JUNB, Jun B proto-oncogene; PRDM1, PR domain containing 1 with ZNF domain; STAT4, signal transducer and activator of transcription 4; FOS, FBJ murine osteosarcoma viral oncogene homolog; ETS2, v-ets avian erythroblastosis virus E26 oncogene homolog 2; VDR, vitamin D (1,25-dihydroxyvitamin D3) receptor; RARA, retinoic acid receptor alpha; RUNX1, runt-related transcription factor 1. Solid line: activation; dashed line: inhibition.

Article Snippet: Affinity-purified goat anti-mouse IRF1 antibody (catalogue #: AF4715) and rabbit anti-goat IgG secondary antibody (catalogue #: HAF017) were obtained from R&D Systems Europe Ltd., United Kingdom, while rabbit anti-human IRG1 antibody (catalogue #: HPA040143) and normal rabbit IgG (catalogue #: sc-2027) were purchased from Sigma and Santa Cruz Biotechnology, respectively.

Techniques: Binding Assay, Virus, Activation Assay, Inhibition

Journal: PLoS ONE

Article Title: Gene Regulatory Network Inference of Immunoresponsive Gene 1 ( IRG1 ) Identifies Interferon Regulatory Factor 1 ( IRF1 ) as Its Transcriptional Regulator in Mammalian Macrophages

doi: 10.1371/journal.pone.0149050

Figure Lengend Snippet: Transcription factors gene essentiality metric scores.

Techniques:

Journal: PLoS ONE

Article Title: Gene Regulatory Network Inference of Immunoresponsive Gene 1 ( IRG1 ) Identifies Interferon Regulatory Factor 1 ( IRF1 ) as Its Transcriptional Regulator in Mammalian Macrophages

doi: 10.1371/journal.pone.0149050

Figure Lengend Snippet: ( A, B ) RAW264.7 cells were transfected with siRNA negative (siRNA NEG) or siRNA specific to Irf1 (siRNA Irf1) 24 hours before treatment and RNA was extracted 2 hours after activation with LPS (10ng/ml). The bars show the mean of 3 biological replicates (± SEM) of ( A ) Irf1 and ( B ) Irg1 mRNA levels measured by real-time PCR normalised with L27 as the housekeeping gene. **p < 0.01, *p < 0.05. ( C ) Proteins were extracted from transfected cells after 4 hours of LPS stimulation. Western blot bands of IRF1, IRG1/CAD and β-ACTIN proteins are shown. ( D, E ) Metabolites were extracted from transfected cells after 4h of LPS stimulation and analysed by GC-MS. Itaconic acid levels in Irf1 silenced cells were calculated as the percentage relative to the non-specific transfected cells in ( D ) control and ( E ) LPS activated cells. Error bars and statistical significance were calculated from 3 biological replicates (± SEM). *p < 0.05.

Techniques: Transfection, Activation Assay, Real-time Polymerase Chain Reaction, Western Blot, Gas Chromatography-Mass Spectrometry, Control

Journal: PLoS ONE

Article Title: Gene Regulatory Network Inference of Immunoresponsive Gene 1 ( IRG1 ) Identifies Interferon Regulatory Factor 1 ( IRF1 ) as Its Transcriptional Regulator in Mammalian Macrophages

doi: 10.1371/journal.pone.0149050

Figure Lengend Snippet: PBMCs-derived macrophages were transfected with siRNA negative (siRNA NEG) or siRNA specific to IRF1 (siRNA IRF1) 24 hours before treatment and RNA was extracted 6 hours after activation with LPS (10μg/ml) in independent donors (D1-D3). The bars show the mean of ( A ) IRF1 and ( B ) IRG1 mRNA levels of 3 technical replicates (± SEM) measured by real-time PCR normalised with L27 as the housekeeping gene. *p < 0.05.

Techniques: Derivative Assay, Transfection, Activation Assay, Real-time Polymerase Chain Reaction

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goat anti mouse irf1 antibody (R&D Systems)

Structured Review

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1) Product Images from "Gene Regulatory Network Inference of Immunoresponsive Gene 1 ( IRG1 ) Identifies Interferon Regulatory Factor 1 ( IRF1 ) as Its Transcriptional Regulator in Mammalian Macrophages"

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